Week 2: DNA Extraction and PCR(Polymerase Chain Reaction)

On Tuesday, July 8, 2014, I extracted my first sample of DNA and actually  analyzed the quality of my sample using a nano drop machine. This machine graphs the absorbable of the DNA sample in millimeters vs. the wavelength in nanometers. I used 1.5 nano liters of my sample and had a concentration of 26.8 nano grams and a quality of 1.89. My mentor, Dr. Julia Stevens said this was excellent for my first DNA extraction. I also performed my first PCR on my DNA sample. PCR is used to copy DNA. We used a total of 8 samples, four for the bacteria mixture and four for the fungi mixture. There were also two negative controls. After created the mixture, we placed our tubes in the PCR machine. The pictures below some of these procedures.imageimage